D. B. Ellis, G. Lepage
May 1, 1966
Citations
0
Influential Citations
12
Citations
Quality indicators
Journal
Cancer research
Abstract
Summary The metabolism of 9-/3-D-xylofuranosyladenine (xyl-A) has been studied in several normal and neoplastic mouse tissues. When administered to normal mice at a dose level of 50 mg/kg for 5 days, xyl-A caused a 20% loss in body weight but had no effect on the blood leukocyte level, and no other toxic manifesta tions were apparent. Studies with radiocarbon-labeled xyl-A demonstrated that it rapidly appeared in the blood following i.p. injection, was distributed throughout the tissues, and was rapidly excreted in the urine as xylosyl hypoxanthine. Approxi mately 60% of the administered dose was excreted in the urine from either normal or tumor-bearing mice within 3 hr. No evi dence for any cleavage of xyl-A could be detected, although deamination occurred rapidly. It was demonstrated that the drug was readily phosphorylated, primarily to the triphosphate level, by both normal and neoplastic tissues. In all tissues and tumors studied, relatively large amounts of xylosyl hypoxanthine were found along with varying amounts of the phosphorylated compounds. Xylosyl hypoxanthine was rapidly eliminated from the tumor cells, whereas the nucleotide form of the drug was still present in relatively high concentrations 3 hr after an i.p. injec tion of the drug.