Zhu Lijun, Shanshan Sun, Huang Yanxi
Apr 1, 2018
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Influential Citations
7
Citations
Quality indicators
Journal
Biomedical Chromatography
Abstract
A clear understanding of the metabolism of Traditional Chinese Medicine (TCM) is extremely important in their rational clinical application and effective material foundation research. A novel and reliable strategy was performed to find more metabolites of paeoniflorin, determine the metabolites of total paeony glucosides (TPG) by means of determining those metabolites of paeoniflorin, and compare the metabolism differences between paeoniflorin and TPG by intragastric administration. This strategy was characterized as follows: firstly, the rats were divided into two groups (the paeoniflorin group and the TPG group) to find different metabolism mechanism between paeoniflorin and TPG; secondly, UPLC-FT-ICR MS and UPLC-Q-TOF MS2 were applied to obtain accurate molecular weight and structural information respectively; thirdly, the metabolites were tentatively identified by a combination of data-processing methods including mass defect screening, characteristic neutral loss screening and product ion screening; finally, a comparative study was employed in the metabolism of paeoniflorin and TPG. Based on the strategy, eighteen metabolites of paeoniflorin (including four new compounds) and eleven metabolites of TPG (including two new compounds) were identified respectively. In all of the identified metabolites of paeoniflorin, two metabolites in rat plasma, four metabolites in rat urine and six metabolites in rat feces were found for the first time after paeoniflorin administration, respectively. The results indicated that the hydrolyzation of the ester bond and glucosidic band and the conjugation with glucuronide were the major metabolic pathways of paeoniflorin. It has been the first time to detect the metabolites of paeoniflorin and TPG in rats plasma, urine and feces after intragastric administration. The results may contribute to a better understanding of the metabolism mechanism and providing a scientific rationale for researching the material basis of paeoniflorin and TPG in vivo.