Xinyuan Xu, J. Diao, Xinru Wang
May 1, 2012
Citations
0
Influential Citations
12
Citations
Quality indicators
Journal
Pesticide Biochemistry and Physiology
Abstract
Abstract We investigated the metabolic kinetics and toxicity of ethofumesate (ETO) in rat and chicken hepatocytes using a chiral high-performance liquid chromatographic (HPLC) method. The metabolic of ETO in rat hepatocytes was enantioselective, whereas it was not in chicken hepatocytes. The T 1/2 of (−)-ETO was about two times longer than that of (+)-ETO after the rat hepatocytes had been incubated with 20 μM rac -ETO. There was no chiral conversion or transformation during their incubation with the hepatocytes. Toxicity differences were observed between the two enantiomers of ETO, reflected in their EC 50 values in rat and chicken hepatocytes. The stereoselective cytotoxicity of the two enantiomers was opposite in rat and chicken hepatocytes. We have developed a method of studying the toxicokinetics and cytotoxicity of chiral agrochemicals in hepatocytes isolated from mammals (rats) and chicken. The data presented here allow a more thorough understanding of this pesticide and should be useful in its full environmental assessment.