T. L. Laanio, P. C. Kearney, D. D. Kaufman
Sep 1, 1973
Citations
0
Influential Citations
27
Citations
Journal
Pesticide Biochemistry and Physiology
Abstract
Abstract The microbial metabolism of N 3 ,N 3 -diethyl 2,4-dinitro-6-trifluoromethyl- m -phenylenediamine (dinitramine), N-sec -butyl-4- tert -butyl-2,6-dinitroaniline (A-820), N-n -propyl- N -cyclopropylmethyl-4-trifluoromethyl-2,6-dinitroaniline (CGA-10832), and α,α,α-trifluoro-2,6-dinitro- N,N -dipropyl- p -toluidine (trifluralin) by Aspergillus fumigatus Fres., Fusarium oxysporum Schlecht and Paecilomyces sp. was investigated. The dinitrodiamine and dinitroanilines were most readily metabolized by Paecilomyces sp. The metabolism of dinitramine was examined in detail, and four metabolites were isolated: N 3 -ethyl 2,4-dinitro-6-trifluoromethyl- m -phenylenediamine; 2,4-dinitro-6-tri-fluoromethyl- m -phenylenediamine; 6-amino-1-ethyl-2-methyl-7-nitro-5-trifluoromethylbenzimidazole and 6-amino-2-methyl-7-nitro-5-trifluoromethylbenzimidazole. The presence of a dinitramine-degrading enzyme system in A. fumigatus was demonstrated. The enzyme dealkylates dinitramine and requires NADPH and Fe 2+ as cofactors.