M. Hamberg, B. Samuelsson
Feb 25, 1971
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0
Influential Citations
63
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Journal
The Journal of biological chemistry
Abstract
Abstract 11α,15-Dihydroxy-9-ketoprost-5-enoic acid formed from prostaglandin E2 with the soluble fraction of guinea pig liver homogenates was shown to have the l configuration at C-15. By incubation of prostaglandin E2 labeled with deuterium (inter alia) at C-15 and subsequent mass spectrometric analysis of the 11α,15l-dihydroxy-9-ketoprost-5-enoic acid formed, it was found that the latter compound was not formed by direct reduction of the Δ13 double bond of prostaglandin E2. On the basis of this finding, as well as on results of incubations of tritium-labeled 11α-hydroxy-9,15-diketoprost-5-enoic acid, 11α,15l-dihydroxy-9-ketoprost-5-enoic acid, and prostaglandin F2α, pathways in the metabolism of prostaglandin E2 in guinea pig liver were established.