T. Itoh, S. Itagaki, Yoshinobu Sumi
May 1, 2005
Citations
1
Influential Citations
28
Citations
Quality indicators
Journal
Cancer Chemotherapy and Pharmacology
Abstract
PurposeThe aim of this study was to investigate the transport mechanisms of transporters that contribute to the intestinal uptake of 7-ethyl-10-hydroxycamptothecin (SN-38).MethodsHuman intestinal epithelial Caco-2 cells were used to investigate the mechanistic basis of transepithelial uptake of SN-38. We investigated the characteristics of SN-38 uptake into Caco-2 cells. The effects of baicalin and sulfobromophthalein (BSP) on the uptake of SN-38 by Caco-2 cells were examined.ResultsUptake of SN-38 was significantly reduced at 4°C. Baicalin inhibited the uptake of SN-38 in a concentration-dependent manner. BSP significantly reduced the uptake of SN-38. However, probenecid, pravastatin and grepafloxacin did not affect the uptake of SN-38.ConclusionsThe results suggest that a specific transport system mediates the uptake of SN-38 across the apical membrane in Caco-2 cells.