W. Xue
2002
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0
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Citations
Journal
Progress in Biochemistry and Biophysics
Abstract
Cyanogen bromide has been widely used as an activator to some resins while preparing affinity chromatography column and immobilized enzymes. Nevertheless, it is a hazard poison with a volatilization feature, which should be carefully used. Here, 1,1′ carboxyl diimidazole is used as an activator to Sepharose CL 6B , in order to immobilize earthworm fibrinlytic enzyme Ⅲ 1 (EFE Ⅲ 1), m aminophenylboronic acid and to detect glycated D glyceraldehyde 3 phosphate dehydrogenase (GAPDH), with a low poison, a high coupling rate and a convenient process.