P. Sundaram
Nov 27, 1974
Citations
0
Influential Citations
29
Citations
Journal
Biochemical and biophysical research communications
Abstract
Abstract 1. 1) A new mild method for the covalent coupling of enzymes to COOH group bearing insoluble polymers such as CM-cellulose and CM-Sephadex is described in this paper. N-ethoxycarbonyl-2-ethoxy 1,2-dihydroquinoline (EEDQ) is used to activate the polymer before reacting with the enzyme. 2. 2) Optimal amounts of enzyme are coupled when the polymer is activated at pH 4. 3. 3) Large amounts of protein retaining appreciably high specific activity may be immobilized by this procedure.