Shi Ren-bing
2007
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Journal
Journal of Beijing University of Traditional Chinese Medicine
Abstract
Objective To establish a method for determinating the total chromone in the chromone fraction of Saposhnikovia divaricata(Turcz.)Schischk.Method The content of total chromone in chromone fraction of Saposhnikovia divaricata(Turcz.)Schischk.was determined by the chromatoptometry of molybdenum phosphotungstate-lithium sulfate:hamaudol was taken as the reference substance and molybdenum phosphotungstate-lithium as the developer.The procedure was developed in buffer with pH value of 10.6.Result Hamaudol had a good linear relationship with the absorbance in the range of 8 to 56 μg,and the linear regression equation was Y=0.203 4X-0.002 1(r=0.999 8).The average recovery for hamaudol was 98.82% with RSD=1.97%(n=6).Conclusion The content determination of 3 batches of sample shows that the method is simple,convenient and accurate,and can be used for the content determination of the total chromone in the chromone fraction of Saposhnikovia divaricata(Turcz.)Schischk.