B. Blessington, A. Beiraghi
1991
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Chirality
Abstract
A rapid, quantitative, enantioselective HPLC method, using N,N′-dibenzylethylenediamine as internal standard, for the analysis of the three stereoisomers of the antituberculosis drug ethambutol and 2-aminobutan-1-ol is described. The derivatisation and chromatographic procedures and their reproducibility, durability, and sensitivity are discussed. Application to assays of raw material, tablet content, and synthetic reactions are illustrated. The method involves perbenzoylation, in pyridine, followed by direct HPLC on a “Pirkle covalent bonded column” containing a 3,5-dinitrobenzoyl-D-phenylglycine as chiral stationary phase (CSP) using hexane/IPA as mobile phase and detection at 230 nm.