D. Samuel, P. Amlot, R. Abuknesha
Jul 16, 1985
Citations
0
Influential Citations
6
Citations
Journal
Journal of immunological methods
Abstract
A simple and efficient method is described to overcome the difficulty in radiolabelling ovalbumin and is applicable to any protein. Coumarin-3-acetic acid-N-OH-succinimidyl ester (CASE) binds directly to ovalbumin in aqueous solution to form covalent bonds with free amino groups. The attached CASE residues can then be radiolabelled by the chloramine-T method. This offers an inexpensive alternative to the Bolton and Hunter reagent with the advantage that CASE is fluorescent. This property, apart from direct use as a fluorescent tag, allows one to monitor the conjugate before radiolabelling. Below a conjugation ratio of 9:1, CASE-ovalbumin retained full antigenic identity with ovalbumin.