F. M. Rossi, J. Kao
Jul 29, 1997
Citations
0
Influential Citations
25
Citations
Journal
Bioconjugate chemistry
Abstract
An efficient preparative method for separating 5- and 6-carboxyfluorescein is presented. 6-Carboxyfluorescein dipivalate is isolated as its diisopropylamine salt, which can be converted to the free acid or used directly in coupling reactions. The 5-isomer is isolated from the acidified mother liquor. Isomerically pure carboxyfluoresceins are prepared by hydrolysis of the corresponding dipivalates.