Ying Wang, C. Calas‐Blanchard, M. Cortina-Puig
Jun 1, 2009
Citations
1
Influential Citations
20
Citations
Journal
Electroanalysis
Abstract
A simple and sensitive electrochemical method for the determination of antioxidant capacity has been studied. 4-hydroxybenzoic acid (4-HBA) was used as a trapping agent for photogenerated .OH radicals, leading to 3,4-dihydroxybenzoic acid (3,4-DHBA). Square-wave voltammetry was used to quantify the amount of 3,4-DHBA formed from the reaction between 4-HBA and .OH. Addition of antioxidants induced the competition between 4-HBA and antioxidants toward .OH elimination, resulting in a decrease of the measured current. The IC50 for different standard antioxidants was calculated and expressed in Trolox equivalent antioxidant capacity (TEAC). The proposed method was successfully compared to a fluorimetric one.