B. S. Martinis, M. Bianchi
Aug 1, 2002
Citations
1
Influential Citations
34
Citations
Journal
Pharmacological Research
Abstract
Abstract The compound 8-hydroxy-2′-deoxyguanosine (8-OHdG), one of the major oxidative DNA base products, is a commonly used biomarker of oxidative DNA damage. The routine measurement of 8-OHdG in biologic samples, and especially in urine, is inherently a challenging analytical problem due to the low levels of the analyte and the complexity of the sample. In this study, a simple and rapid procedure based on one step solid phase extraction (SPE) followed by high-performance liquid chromatography with electrochemical detection (HPLC-ECD) analysis is described. The methodology proposed resulted in a recovery of 74.5±12% for urinary 8-OHdG with a detection limit of 5.0 μ g l −1 .