R E London
1991
Citations
8
Influential Citations
148
Citations
Quality indicators
Journal
Annual review of physiology
Abstract
Accurate and reproducible methods for the measurement of cytosolic free magnesium ion concentration, Mgj, are required in order to assess its physiologic role. This review covers two methods, in vivo nuclear magnetic resonance (NMR) and fluorescence spectroscopy, which have provided useful means for the determin�ation of Mgj. The first section contains a general discussion of the extraction of Mgj values from NMR or fluorescence data for magnesium ion chelators. Subsequent sections cover the use of endogenous NMR magnesium chelators, exogenous NMR Mgj indicators, and fluorescent Mgj indicators. Two recent reviews (2, 15) covered Mgj determinations based on ATP measurements, but did not cQver more recently developed fluorinated or fluorescent indicators. Other methods are covered in References 2 and 34. With the availability of new Mgj indicators, our understanding of the role of magnesium in metabolic regulation and in the etiology of disease will un doubtedly increase in the near future.