Yuri Tanioka, E. Miyamoto, Y. Yabuta
Jul 16, 2010
Citations
2
Influential Citations
31
Citations
Journal
FEBS Letters
Abstract
To clarify the physiological function of pseudovitamin B12 (or adeninylcobamide; AdeCba) in Spirulina platensis NIES‐39, cobalamin‐dependent methionine synthase (MS) was characterized. We cloned the full‐length Spirulina MS. The clone contained an open reading frame encoding a protein of 1183 amino acids with a molecular mass of 132 kDa. Deduced amino acid sequences of the Spirulina MS contained critical residues identical to cobalamin‐, zinc‐, S‐adenosylmethionine‐, and homocysteine‐binding motifs. The recombinant Spirulina enzyme showed higher affinity for methyladeninylcobamide than methylcobalamin as a cofactor. These results indicate that Spirulina cells can utilize AdeCba synthesized as the cofactor for MS.