Y. Kakimoto
Jul 25, 1971
Citations
0
Influential Citations
38
Citations
Quality indicators
Journal
Biochimica et biophysica acta
Abstract
Abstract 1. 1. Incorporation of Me-14C S-adenosylmethionine into arginine and lysine residues of proteins was studied using extracts of rat brain and liver. The amino acids were methylated to form NG,NG- and NG,N′G-dimethyl- and NG-monomethyl-arginine and Ne-mono-, di- and trimethyllysine residues. The labelled products were identified using ion-exchange and paper chromatography, paper electrophoresis and chemical degradation. 2. 2. The methylation products of arginine residues have been tentatively identified as NG-methyl- and Nα,NG-dimethylarginines in other laboratories, but this is now proved to be incorrect. The compound proposed as NG-methylarginine is a mixture of NG,NG- and NG,N′G-dimethylarginines and that supposed to be Nα,NG-dimethylarginine is most likely NG-methylarginine.