J. Daly, D. Jerina, J. Farnsworth
Apr 1, 1969
Citations
0
Influential Citations
38
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Abstract Acetanilide-4- 2 H is converted to 4-hydroxyacetanilide-3- 2 H during incubation with liver microsomal preparations. The degree of migration and retention of deuterium in this reaction, previously shown to depend on the pH of the media, is also dependent upon the species, strain, and sex of the animal used as the source of microsomal preparations. Pretreatment of animals with phenobarbital causes an increase in the retention of deuterium in 4-hydroxyacetanilide produced from 4-deuteroacetanilide while pretreatment with benzpyrene or 3-methylcholanthrene causes a decrease in the retention. The effect of induction on the migration and retention of deuterium and of tritium is unique to acetanilide-4- 2 H, and is not observed during hydroxylation of anisole-4- 2 H, chlorobenzene-4- 2 H, or 4-fluorobiphenyl-4′- 2 H. It is proposed that two “acetanilide hydroxylases” exist in varying amounts in different species, and that one, characterized by exhibiting high retentions of deuterium in the hydroxylation of acetanilide-4- 2 H, is induced by phenobarbital, while the other, characterized by exhibiting low retentions of deuterium in the same reaction, is induced by 3-methylcholanthrene and benzpsyrene.