K. Takai, Shuhei Okumura, K. Hosono
Mar 19, 1999
Citations
2
Influential Citations
29
Citations
Journal
FEBS Letters
Abstract
5‐Methoxyuridine was introduced into the first position of the anticodon of the unmodified form of tRNA1 Ser from Escherichia coli. The codon reading efficiencies of this tRNA (tRNA(5‐methoxyuridine UGA)) relative to those of the unmodified counterpart (tRNA(UGA)) were measured in a cell‐free translation system. tRNA(5‐methoxyuridine UGA) was more efficient than tRNA(UGA) in the reading of the UCU and UCG codons and was less efficient in the reading of the UCA codon. Thus, the single modification of U to 5‐methoxyuridine can enhance the wobble readings.