T. Yamashina, H. Hirata
Jun 20, 1987
Citations
0
Influential Citations
1
Citations
Journal
Journal of Japan Oil Chemists Society
Abstract
The reactions of amino groups in amino acids and proteins with sodium 2, 4, 6-trinitrobenzenesulfonate (TNBS·Na) in the presence of sodium sulfite (Na2SO3) were studied at pH 10.1. Determination of the amino groups was performed by following the absorbance A (420 nm) of the Meisenheimer complex. A good linear relationship between A and the concentrations of amino compounds was observed. The reactions in a cuvette showed good agreement with those in the dark. Based on these observations, a modified method for determining amino groups by TNBS·Na in the presence of Na2SO3 is proposed, and several comments are described.