Liu Yuan
2007
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Influential Citations
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Quality indicators
Journal
Carcinogenesis,Teratogenesis and Mutagenesis
Abstract
BACKGROUND AIM:Nitrofen is a herbicide used to establish the pulmonary hypoplasia model.The current study was performed to explore the effects and mechanisms of nitrofen on proliferation and apoptosis of cultured type II pneumocytes.MATERIALS AND METHODS:Type II pneumocytes A549 were treated with 20,40 and 80 μmol/L of nitrofen.Cellular proliferation was measured by MTT colorimetry,colony formation assay.Western blot and real-time RT-PCR were performed to detect the PCNA expression.Apoptosis was assayed by TUNEL labeling and acridine orange-ethidium bromide staining.Expression of apoptosis-related genes was detected by western blot.RESULTS:Compared with controls,administration of 20,40 and 80 μmol/L nitrofen for 12-48 hrs resulted in 12.43%-71.73% decrease in proliferation(P0.01),and 79.2% decrease in colony formation(P0.01) of A549 cells.The PCNA protein and mRNA within nitrofen-treated cells were reduced in a time-and dose-dependent manner(P0.01).Partial nitrofen-treated A549 cells presented morphological changes of apoptosis,with apoptotic rates of 8.0%-22.5%(P0.01),which could not be abolished by the pan caspase inhibitor zVAD-fmk.The expression of anti-apoptotic Bcl-xL was significantly down-regulated(P0.01).CONCLUSION:Nitrofen decreased proliferation of cultured type II pneumocytes associated with down-regulation of PCNA,and induced apoptosis associated with suppressed Bcl-xL expression.