R. Ansell, K. Mosbach
Nov 7, 1997
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Influential Citations
92
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Quality indicators
Journal
Journal of Chromatography A
Abstract
Methacrylic acid (MAA)-trimethylolpropane trimethacryl ate (TRIM) copolymer beads imprinted with tert.-butoxy-carbonyl-l-phenylalanine (Boc-l-Phe) have been prepared by suspension polymerisation in a perfluorocarbon liquid. The suspension polymerisation procedure has been adapted to work with a number of different porogenic solvents, and with UV initiated or thermal initiated polymerisation. The resulting polymers have been evaluated as stationary phases for the chromatographic separation of Boc-d,l-Phe. Polymer beads of average diameter 8–25 μm packed into 100×4.6 mm chromatography columns were used with a mobile phase of dichloromethane-acetic acid (99:1, v/v) at flow-rates up to 5 ml min−1. A polymer prepared with 1,2-dichloroethane as porogenic solvent (average bead diameter 15.1 μm) was capable of the baseline separation of 100 μg Boc-d,l-Phe at a flow-rate of 0.5 ml min−1.