Y. Lee, S. Bae, Ji Yea Kim
Nov 28, 2019
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0
Influential Citations
17
Citations
Quality indicators
Journal
Journal of microbiology and biotechnology
Abstract
Loliolide is one of the most ubiquitous monoterpenoid compounds found in algae, and its potential therapeutic effect on various dermatological conditions via agent-induced biological functions including anti-oxidative and anti-apoptotic properties was demonstrated. Here, we investigated the effects of loliolide on hair growth in dermal papilla (DP) cells, the main dermal components regulating hair growth and loss conditions. To investigate the effect of loliolide on hair inductive properties, we used a three-dimensional (3D) DP spheroid model that mimics the in vivo hair follicle system. Biochemical assays showed that low doses of loliolide increased the viability and spheroid size of 3D DP spheroids in a dose-dependent manner. This result correlated with increases in expression levels of hair growth-related autocrine factors including VEGF, IGF-1, and KGF. Immunoblotting and luciferase-reporter assays further revealed that loliolide induced AKT phosphorylation, and this effect led to stabilization of β-catenin which plays a crucial role in hair inductive properties of DP cells. Further experiments showed that loliolide increased the expression levels of the DP signature genes, ALP, BMP2, VCAN, and HEY1. Furthermore, conditioned media from loliolide-treated DP spheroids significantly enhanced proliferation and the expression of hair growth regulatory genes in keratinocytes. These results suggested that loliolide could function in hair growth inductivity of DP cells via the AKT/ β-catenin signaling pathways.