M. Noelken
May 1, 1980
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0
Influential Citations
1
Citations
Journal
Analytical biochemistry
Abstract
Abstract Phenethylbiguanide ( N -(2-phenylethyl)imidodicarbonimidic diamide) hydrochloride was shown to be a potent denaturing agent, according to optical rotation measurements, which at a concentration of 1.17 m yielded the proper molecular weight of reduced-carboxymethylated hen's egg lysozyme as determined by osmotic pressure measurements. The conformation of the unfolded molecule was identical to that in 6 m guanidine hydrochloride, as was shown by intrinsic viscosity measurements. The relatively low density of the solvent, 1.06 g/ml, recommends it for use in sedimentation-equilibrium studies. It would be possible to use rotor speeds 15% lower than those required when the more commonly used solvent, 6 m guanidine hydrochloride (density ∼1.14 g/ml) is used.