Zsuzsanna Eke, K. Torkos
May 1, 2004
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Influential Citations
11
Citations
Journal
Microchemical Journal
Abstract
Abstract N , N -dimethyl-trimethylsilyl-carbamate, a commercially available silylating agent, has been tested in the derivatization of trichothecenes for the first time. Its reaction with alcohols is an autocatalytic, non-equilibrium process with volatile by-products. As a consequence of these advantageous characteristics N , N -dimethyl-trimethylsilyl-carbamate proved to be easily utilizable and effective for the derivatization of all studied trichothecenes, namely deoxynivalenol (DON), nivalenol (NIV) and 4,15-diacetoxyscirpenol (DAS). During testing also, optimum conditions (temperature: 15 °C, reaction time: 35 min) were established for the trimethylsilylation and for the gas chromatographic determination of the above-mentioned three trichothecenes. The linearity of the detector response was found to be proper in the 5–1100 ng range. (Given in the grams of trichothecene equivalent to the derivatives injected). The limit of detection is less than 1 ng for each of the studied trichothecenes. Relative standard deviations of the peak heights ranged from 0.75 to 4.32%.