B. Belisle, A. Abo
Aug 25, 2000
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Journal
The Journal of Biological Chemistry
Abstract
The N-formyl peptide receptor is a G protein-coupled transmembrane receptor involved in stimulating a variety of differential responses in neutrophils including chemotaxis, degranulation, superoxide production, transcriptional activation, and actin reorganization. Although it is known thatN-formyl-Met-Leu-Phe induces actin reorganization, the sequence of events from the receptor to the actin cytoskeleton is not well characterized. To study the signaling pathway from theN-formyl peptide receptor to the actin cytoskeleton, we developed a model system utilizing microinjection techniques with a nonhematopoietic cell line. An expression vector coding for theN-formyl peptide receptor was microinjected into porcine aortic endothelial cells and stimulated withN-formyl-Met-Leu-Phe to induce actin reorganization and membrane ruffling. The receptor-mediated signal was blocked by pertussis toxin and by a dominant negative Rac-N17, indicating the involvement of Giα subunit and the small guanosine triphosphatase Rac, respectively. Moreover, Gβγ subunits and membrane targeted forms of phosphatidylinositol (PI) 3-kinase α were sufficient to induce similar actin reorganization, and coexpression of various mutants of PI 3-kinase with theN-formyl peptide receptor identified a link to class Ia PI-3 kinase-mediated actin reorganization.