J. W. Bardenheier, G. Popják
Feb 7, 1977
Citations
1
Influential Citations
8
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Abstract Commercial [5-14C]mevalonate is shown to contain several radioactive impurities, which give artifactually high amounts of Hyamine bound, volatile acidic radioactivity when incubated with killed or living rat renal cortex slices, as compared with [5-14C]mevalonate purified either by liquid-liquid partition chromatography or through the enzymically generated R -5-phospho-[5- 14 C]mevalonate by ion-exchange chromatography. The artifactual 14 CO 2 results were not diluted by incubation with increasing amounts of unlabelled mevalonate, whereas the 14 CO 2 and [ 14 C ]cholesterol produced by rat renal cortex slices incubated with purified [5-14C]mevalonate were both diluted to the same extent by unlabelled mevalonate. It is concluded that R [5- 14 C]mevalonate is genuinely oxidized to 14 CO 2 in vitro , and that purification of substrate before its use is necessary. Production of 14 CO 2 and various [ 14 C ]lipids from purified [5-14C]mevalonate, as a function of time and substrate concentration, by renal cortex and liver slices, is described.