Xu Jin, Jian-qing Zhang, Yun Li
Jan 27, 2021
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Influential Citations
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Journal
Journal of separation science
Abstract
A key segment in medicinal plant authentication is the establishment of quality markers that embody the intrinsic metabolites difference independent of instruments and experiment conditions. A strategy integrating nontargeted metabolomics and multi-criteria decision making model for robust quality markers discovery is presented and applied to authenticate Ophiopogon japonicus (L. f.) Ker-Gawl. First, an ultra-performance liquid chromatography/quadrupole time-of-flight MSE approach was established for global metabolites profiling and identification. Second, multivariate statistical analysis was performed to explore potential quality markers of different origins of ophiopogonis radix. Third, potential quality markers were ordered and filtered by multi-criteria decision making model to infer robust quality markers and further validated in different instruments and experiment conditions by validation model. Forth, The validation model using the robust quality markers managed to discriminate the origins of ophiopogonis radix samples procured from the herbal markets. Consequently, 2 robust quality markers, cixi-ophiopogon B and ophiopogonin D were discovered and further validated on different instruments and experiment conditions. This integrated strategy provided a practical solution for reliable and convenient authentication of geo-authentic herb. This article is protected by copyright. All rights reserved.