P. Watthaisong, Pornkanok Pongpamorn, P. Pimviriyakul
Jun 24, 2019
Citations
0
Influential Citations
13
Citations
Journal
Angewandte Chemie
Abstract
The flavin-dependent monooxygenase, HadA, catalyzes the dehalogenation and denitration of the toxicants, nitro- and halogenated phenols, to benzoquinone. The HadA reaction can be applied in one-pot reactions towards the de novo synthesis of D-luciferin by coupling with D-Cys condensation. D-luciferin, a valuable chemical widely used in biomedical applications, can be used as a substrate for the reaction of firefly luciferase to generate bioluminescence. As nitro- and halogenated phenols are key indicators of human overexposure to pesticides commonly used worldwide and indicators of pesticide contamination, the technology provides a sensitive and convenient tool for improved biomedical and environmental detection at ppb sensitivity in biological samples without the requirement for any pre-treatment. This newly developed methodology provides the dual-pronged advantage of waste biodetoxification to produce a valuable chemical as well as a smart detection tool for environmental and biomedical detection.