J. Cavadore, G. Nota, G. Prota
Aug 1, 1974
Citations
0
Influential Citations
11
Citations
Journal
Analytical biochemistry
Abstract
Pivalyl chloride and benzoyl chloride are utilized as reagents for the N-terminal analysis of polypeptide chains. Pivalyl and benzoyl derivatives obtained are analyzed by gas-liquid chromatography using glass capillary columns. The chromatographic resolution of the most common amino acid derivatives allows a quantitative estimation of the N-terminal residues even in the case of complicated peptide mixtures.