J. Fischer, M. C. Jost
Jul 1, 1969
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0
Influential Citations
44
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Journal
Molecular pharmacology
Abstract
Using a technique based on the relaxation phenomenon of nuclear magnetic resonance, the interaction of a drug and its receptor site has been directly observed in an intact cellular system. The NMR spectrum of epinephrine is altered by the presence of liver cells in a manner characteristic of a binding interaction. The spectral changes permit certain conclusions to be drawn about time nature of the binding. Dichloroisoproterenol, a beta-adrenergic blocking agent, competitively inhibits the changes in the epinephrine spectrum while its own spectrum exhibits evidence of binding.