M. Nowak, K. Zawadzka, K. Lisowska
Apr 12, 2020
Citations
0
Influential Citations
18
Citations
Journal
Chemosphere
Abstract
Methylisothiazolinone is a commonly used biocide that is released into natural environments. In this work, the ability of the fungal strain Phanerochaete chrysosporium DSM 1556 to biotransform this compound was evaluated. The tested strain was able to remove MIT (at concentrations 50 μg L-1 and 30 mg L-1) from the growth medium with the efficiency 90% after the first 6 h and 100% after 12 h of incubation. Moreover, for the first time, qualitative LC-MS/MS and GC-MS analysis showed monohydroxylated and dihydroxylated methylisothiazolinone and N-methylmalonamic acid as the main products of fungal biodegradation. The ecological toxicity of the tested biocide and its derivatives was also evaluated by using an acute toxicity test with Daphnia magna. An approximately 90% decrease in the toxicity of metabolites formed in the P. chrysosporium culture was noticed. The concentration of MIT in soil and water samples collected in Poland was assessed for the first time. The analysis showed that the selected locations in Poland are contaminated by MIT in the range from 1.04-10.08 μg L-1.