Matteo Calcagnile, P. Alifano
Jun 15, 2022
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Influential Citations
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Journal
The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance
Abstract
: Spiramycin is a 16-membered macrolide used in human medicine as an antibacterial and antiparasitic agent. Spiramycin is effective against bacterial pathogens including Gram-positive and Gram-negative bacteria. In contrast, Pseudomonas aeruginosa is intrinsically resistant to macrolides including azithromycin and spiramycin. Despite the results of in vitro susceptibility tests, interest in macrolides in the treatment of some pseudomonal infections arose from both clinical and preclin-ical studies. For this reason, macrolides have drawn attention as adjunct therapy against chronic and/or biofilm-mediated P. aeruginosa infections. While most of the studies on the antivirulence activity of macrolides focus on erythromycin and its derivative azithromycin, there is no information on spiramycin. We used in vitro methods to test the ability of spiramycin to inhibit certain phenotypic features or traits associated with virulence in P. aeruginosa . Moreover, we tested spiramycin using Galleria mellonella as in vivo model.