K. Yamashita, Y. Tachibana, A. Kobata
Aug 1, 1977
Citations
1
Influential Citations
32
Citations
Journal
Archives of biochemistry and biophysics
Abstract
Abstract Three oligosaccharides which have lacto-N-hexaose as their core structures are isolated by borate paper electrophoresis. These sugars occur in the milk of secretors but are completely absent in the milk obtained from nonsecretor women. By sequential glycosidase digestion and by methylation analysis, their structures were elucidated as Fucαl → 2 Gal/β1 → 3GlcNAcβ1 → 3[Galβ1 → 4GlcNAcβ1 → 6] Galβ1 → 4 Glc (fucosyllacto-N-hexaose I), Fucαl → 2Galβ1 → 3GlcNAcβ1 → 3 [Galβ1 → 4(Fucα1 → 3)GlcNAcβ1 → 6]Galβ1 → 4Glc (difucosyllacto-N-hexaose I), and Fucαl → 2Galβ1 → 3GlcNAcβ1 → 3[NeuAcα2 → 6Galβ1 → 4GlcNAcβ1 → 6]Galβ1 → 4 Glc (sialyl fucosyllacto-N-hexaose I). A-enzyme, which is responsible for the formation of blood group A determinant, can transfer N-acetylgalactosamine from UDP-GalNAc to both fucosyllacto-N-hexaose I and difucosyllacto-N-hexaose I, but not to sialyl fucosyllactoN-hexaose I.