Zhou Dan
2012
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Journal
Food Science
Abstract
Derivatization reaction is very important for the determination of sec-butylamine.For the purpose of establishing a gas chromatography-electron capture detection(GC-ECD) method for determining sec-butylamine,the optimal derivatization reaction conditions were studied by response surface methodology.Based on the results of one-factor-at-a-time experiments,a central composite design was used to investigate the pairwise interaction effects of reaction time,temperature and derivatization reagent(acetonitrile containing 0.05μL/mL pentafluorobenzaldehyde) amount on sec-butylamine recovery.The optimal derivative temperature,derivative duration and derivatization reagent amount were found to be 22.42 ℃,31.72 min and 1.25 mL,respectively.