Liu Chun-ye
2012
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Journal
Chemical Research and Application
Abstract
Labeling of carboxyl fractions is important to the research of traditional Chinese medicine.It can improve the detection sensitivity and perfect the pharmacokinetics studying of Chinese medicine.The labeling method and its evaluation system are presentedin this paper.In the work,trisodium 8-aminopyrene-1,3,6-trisulfonate(APTS)and chlorogenic acid(CGA)were chosen to forma labeling system.If EDC and NHS exist,the labeling reaction can take placeat room temperature.Experimental resultsshowthat the prefer buffer system was NaH2PO4-Na2HPO4(0.1mol/L,pH5.50).CGA reacted with EDC and NHS in above buffer for 4 h at room temperature.The concentration of EDC and NHS was 5 mmol/L and 0.33 mmol/L,respectively.Then,APTS(concentration ratio of CGA and APTS was 1∶5)was added to above reaction systems and continue reacted for 4 h.Conclusions The labling method is not only simple but also sensitive,and can be possibly used in study ofcarboxylfractionof Chinese medicinelabeling.