M. Papisov, A. Maksimenko, V. Torchilin
1985
Citations
0
Influential Citations
31
Citations
Journal
Enzyme and Microbial Technology
Abstract
Abstract The kinetics of 1-ethyl-3-(3′-dimethylaminopropyl)-carbodiimide interaction with carboxylic groups in low and high molecular weight compounds have been investigated for choosing the optimum conditions for enzyme immobilization on carboxylic carriers. Optimum pH values depended on the pKa of an acid. The ionic strength of the reaction mixture influenced the process of activation with carbodiimide and a decrease in reaction rate accompanied an increase in ionic strength. Optimum conditions for the coupling process with the use of carbodiimide activation are suggested. The process of fibrinolysin immobilization on the soluble copolymer of acrylic acid and acrylamide with carbodiimide as a coupling agent was also studied.