Sanders Junglee, L. Urban, H. Sallanon
Aug 8, 2014
Citations
8
Influential Citations
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Quality indicators
Journal
American Journal of Analytical Chemistry
Abstract
Here, we present an optimization of colorimetric determination of hydrogen peroxide content in plants using potassium iodide. Our method is based on a one step buffer (extraction and reaction) for the determination of H2O2 in different plant tissues and overcomes interference of soluble antioxidant and color background. A particular attention is paid to buffer pH shown to be tissue dependent. With this inexpensive microplate method, it is possible to analyze 12 experimental samples in about 45 min all in triplicates, with blanks, controls and standard curve.