M. Mizell, S. Simpson
1961
Citations
0
Influential Citations
41
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The solvent n-butanol—methyl ethyl ketone—water (2:2:1) with an atmosphere saturated with cyclohexylamine yields excellent separation of mixtures of all the common acids, is readily reproducible, lacks the capriciousness of phenolic systems, and furthermore, imparts distinctive colors to many of the amino acids after ninhydrin treatment. These characteristics colors serve to confirm identification based on control runs for these amino acids and also serve as valuable “landmarks” facilitating the identification of adjacent amino acids that have the customary purple ninhydrin color.