H. Yamada, S. Shimizu, Jong Min Kim
Dec 1, 1985
Citations
0
Influential Citations
23
Citations
Journal
Fems Microbiology Letters
Abstract
A novel metabolic pathway for creatinine, degradation of creatinine to glycine via N-methylhydantoin, N-carbamoylsarcosine and sarcosine in turn, was found to operate in Pseudomonas putida 77. N-Methylhydantoin, sarcosine and glycine were detected as metabolites from creatinine, and enzyme activities responsible for the degradation of creatinine, N-methylhydantoin, N-carbamoylsarcosine and sarcosine were observed in a crude cell extract, while neither creatinine amidohydrolase activity nor creatine were detected at all. It is suggested that creatinine was utilized as a source of both carbon and nitrogen at the same time, and that N-methylhydantoin was also utilized efficiently.