N. Xaus, X. Jorba, S. Calvet
1992
Citations
0
Influential Citations
2
Citations
Journal
Biotechnology Techniques
Abstract
The insect neuropeptide derivative Z-Gly-Gly-Ser-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 has been obtained in good yields by condensation of two peptide fragments through formation of the Tyr5-Ser6 bond by α-chymotrypsin (α-CT). Among the three reaction media examined, reversed micelles and low water containing acetonitrile, but not aqueous-N,N dimethyl-formamide (DMF) solutions, proved to be suitable systems to assist α-chymotrypsin catalysis.