H. Gaertner, A. Puigserver
1988
Citations
0
Influential Citations
39
Citations
Journal
Proteins: Structure
Abstract
Chymotrypsin modified with polyethylene glycol was successfully used for peptide synthesis in organic solvents. The benzene‐soluble modified enzyme readily catalyzed both aminolysis of N‐benzoyl‐L‐tyrosine p‐nitroanilide and synthesis of N‐benzoyl‐L‐tyrosine butylamide in the presence of trace amounts of water. A quantitative reaction was obtained when either hydrophobic or bulky amides of L‐ as well as D‐amino acids were used as acceptor nucleophiles, while almost no reaction occurred with free amino acids or ester derivatives