M. Puiu, Irina Babaligea, Cătălina Olmazu
Nov 15, 2010
Citations
0
Influential Citations
17
Citations
Journal
Biochemical Engineering Journal
Abstract
Abstract A kinetic model able to adequately describe the accumulation of p-topaquinone in peroxidase-mediated oxidation of l -dopa was developed, and the rate constants for both enzymatic and non-enzymatic branch were estimated either experimentally or using a computing program for detailed kinetic simulation. It is demonstrated that the accumulation of p-topaquinone in significant amounts occurred in excess of hydrogen peroxide or during auto-oxidation of l -dopa, but changes in the ratio of initial concentrations of the reactants can reduce considerably the production of this intermediate.