K. Yasumoto, K. Sugiyama
Jun 1, 1980
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0
Influential Citations
5
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Quality indicators
Journal
Agricultural and biological chemistry
Abstract
Glycyl-L-leucine is one of the best substrates for peptide hydrolases in the intestinal mucosa. Its absorption and hydrolysis were investigated in epithelial cells isolated from the rat intestine in the presence of bestatin, a specific inhibitor of certain peptide hydrolases. Bestatin competi-tively inhibited dipeptide hydrolase activities in isolated cells with a Ki value of 10-8M, but noncompetitively inhibited, and less significantly, the dipeptide absorption by isolated cells. At 10-4M bestatin inhibited half the dipeptide absorption, but only minimally inhibited the absorption of constituent amino acids. In the presence of bestatin at substantial concentrations the isolated cells took up a significant amount of the intact dipeptide, which otherwise appeared entirely in the form of free amino acids. These results are interpreted to substantiate a notion that a dual mechanism is operative for the absorption of readily-hydrolysable peptides: the peptide hydrolysis followed by uptake of thereby released amino acids, and the peptide transport followed by cytosolic hydrolysis.