J. Hui
2001
Citations
0
Influential Citations
0
Citations
Quality indicators
Journal
Journal of China Pharmaceutical University
Abstract
AIM The purpose is to establish a reversed phase HPLC method for determination of ampicillin and to study it′s pharmacokinetics in healthy subjects. METHOD S The plasma concentration of ampicillin, a main active metabolite of bac ampicillin, was determined by a HPLC method. The drug was extracted from plasma with perchloric acid. Hypersil C 18, 5 μm,150 mm×4.6 mm I.D. column was used. Mobile phase consisted of 78% 11 mmol/L KH 2PO 4 (pH 4.0)and 22% met hanol. The eluted peaks were detected by UV detector at 231 nm. The pharmacokin etics of bacampicillin in 20 healthy subjects was studied after oral administra tion of 800 mg. RESULTS The recoveries of ampicillin from plasma were larger than 90% and RSD(%) of intra-day and inter-day was smaller th an 15% . Calibration curves were linear over 0.316~20.220 μg/ml (r=0.9998,P0.01 )and the minimum detection concentration was 0.316 μg/ml. After oral administra tion of 800 mg bacampicillin tablet in 20 men, the main pharmacokinetic paramete r s were estimated as follows:T 1/2K 1.13±0.31 h, T max 0.81 ±0.30 h,c max 10.75±2.53 μg/ml, MRT 2.01±0.40 h, and AUC\+6\-0 21.25±4.20 μg·h/ml. CONCLUSION The assay method was convenie nt for clinical study of bacampicillin.