Zhao Aiguo
2013
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Quality indicators
Journal
Traditional Chinese Drug Research and Clinical Pharmacology
Abstract
Objective To establish a HPLC method for the measurement of neogambogic acid concentration in rabbit plasma,and to study the pharmacokinetics of neogambogic acid in rabbits.Methods The plasma samples were collected at diferent time after intravenous injection of single dose of neogambogic acid,and then were acidified with HCl,extracted with ethyl acetate and evaporated for dryness.The residuals were dissolved with mobile phase and injected into Phenomenex Luna C18 column.The mobile phase consisted of acetonitrile-0.1 mol·L-1 ammonium acetate-acetic acid(80 ∶ 20 ∶ 0.1),and was delivered at the flow rate of 1.0 mL·min-1.The elute was detected at UV 355 nm.The column temperature was 30 ℃.The pharmacokinetic parameters were assessed with non-compartment model.Results The standard curve was linear in the range of 0.518~266 mg·L-1(r=0.9995),and the recovery of neogambogic acid was over 90 %.The relative standard intra-day and inter-day deviation was less than 15%.The pharmacokinetic results showed that the drug was rapidly eliminated from plasma and elimination half life(t1/2) was 23.38 min,the apparent volume of distribution was 0.13 L·kg-1.The AUC0-∞ was 1107.00 mg·min·L-1.Conclusion The established HPLC method is suitable for the pharmacokinetic study of neogambogic acid.The elimination of neogambogic acid is fast in rabbits,and the retention time is short.