D. Ferry, M. Rombusch, A. Goll
Apr 9, 1984
Citations
0
Influential Citations
63
Citations
Quality indicators
Journal
FEBS Letters
Abstract
A 1,4‐dihydroypyridine arylazide photoaffinity ligand, [3H]azidopine (50.6 Ci/mmol), has been synthesized. [3H]Azidopine binds reversibly with a K d of 350 pM to guinea‐pig skeletal muscle membranes in the absence of ultraviolet light. The reversible [3H]azidopine binding is inhibited stereoselectively by 1,4‐dihydropyridines, phenylalkylamine Ca2+ channel blockers and La3+. Covalent incorporation into membrane proteins after photolysis was investigated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis. [3H]Azidopine is photoincorporated specifically into a protein of M r∼145 000. The covalent labelling of the M r ∼145 ooo band is inhibited stereoselectively by drugs and cations which block the reversible [3H]azidopine binding. It is suggested that [3H]azidopine is photoincorporated into a subunit of the putative Ca2+ channel.