J. Koukol, C. Chow, B. Vennesland
Aug 1, 1959
Citations
0
Influential Citations
9
Citations
Journal
The Journal of biological chemistry
Abstract
Digitonin has been used successfully to disrupt particulate structures such as mitochondria without loss of their capacity for causing oxidative phosphorylation (1, 2). The present report describes a procedure whereby spinach chloroplasts were extensively disrupted by digitonin extraction to give a preparation of small fragments which could cause an appreciable rate of photophosphorylation. In the presence of these fragments, the lightinduced synthesis of adenosine triphosphate from adenosine diphosphate and orthophosphate was consistently stimulated by added glutathione or coenzyme A. Photophosphorylation by the fragments could also be st’imulated by addition of soluble extracts prepared from whole chloroplasts. Evidence will be presented that the action of the extracts is complex.