H. Kutlu, E. Çömlekçi, H. İzgördü
Jun 1, 2018
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Quality indicators
Journal
ESMO Open
Abstract
Introduction Carmofur (1-hexylcarbamoyl-5-fluorouracil, HCFU, Mifurol) is one of the masked forms of 5-fluorouracil widely used as an antineoplastic agent. The carmofur molecule contains an active core of 5-fluorouracil and hexylcarbamoyl substituent as a transport form, and it helps 5-FU to penetrate into the cell. In the last years the interest in using of 5-fluorouracil derivatives in cancer research was increased. Therefore, the aim of the present study is to evaluate the potential cytotoxic effect of carmofur on the breast cancer cell line, MCF-7. Material and methods MTT assay, was used to detect cytotoxicity. For MTT assay, MCF-7 cells were treated with different concentrations of carmofur for 24 hours in 96-well plates. Incubated cells were quantifiend by spectrophotometry using a plate reader ar 570 nm wave lenght. Viability percentages were calculated from the obtained absorbances and IC50 value was determined. Results and discussions Treatment of MCF-7 cells with different concentrations of carmofur resulted in a significant increase in the cell death in 24 hours. As conclusion, this study has shown that applied dose of carmofur in MCF-7 cells has an cytotoxic effect in concentration dependent manner. Conclusion The calculated IC50 value was low enough for decreasing the viability of breast cancer cells in 24 hours. This result underline the potential cytotoxic effect of carmofur for MCF-7 cells and is a initial further investigation in manner of drug developing for cancer treatment.