Y. Shintani, K. Iwamoto, K. Kitano
Feb 1, 1988
Citations
1
Influential Citations
25
Citations
Journal
Applied Microbiology and Biotechnology
Abstract
SummaryPolyethylene glycol (PEG), a well known fusogen for mammalian cells and microbial or plant protoplasts, markedly stimulated the growth of mammalian cells at low concentration under serum-free or low serum-conditions. Polyvinyl alcohol and polypropylene glycol also stimulated cell growth. A serum-free medíum containing PEG, PEG-86-1, has been established for cultivating a variety of mammalian cell lines. It is also useful for producing an anti-tetanus toxoid human monoclonal antibody by a mouse · human-human heterohybridoma.